The JCB DataViewer is a browser-based application for viewing original image files - from various types of microscopes and gel-documentation systems - associated with JCB articles. It is the first browser-based system for viewing multi-dimensional light microscope image data.

Please note: Data for all articles submitted to the JCB after December 1, 2008 will be peer reviewed. All data for articles submitted before this date were uploaded retrospectively and were not peer reviewed.

Featured Images

FYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate microtubule plus end–directed vesicle transport

Serhiy Pankiv, Endalkachew A. Alemu, Andreas Brech, Jack-Ansgar Bruun, Trond Lamark, Aud Øvervatn, Geir Bjørkøy, Terje Johansen

jcb. 2010. 188:253-269 DOI: 10.1083/jcb.200907015.

(in Figure 3: D)

Movie of GFP-FYCO1 (green) and mCherry-LC3B positive vesicles

FYCO1 colocalizes with LC3B on the rim, but not inside of the vesical structures. HeLa cells co-transfected with GFP-FYCO1, mCherry-LC3B and myc-p62 were imaged by confocal microscopy 48h after transfection.

[ Full Viewer | Article | Figure ]

GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking

Yi Xiang, Yanzhuang Wang

jcb. 2010. 188:237-251 DOI: 10.1083/jcb.200907132.

(in Figure 1: C)

GRASP55 RNAi+GFP

rtTA HeLa cells expressing EGFP using an inducible retroviral expression system were transfected with RNA oligos for human GRASP55. Doxycycline was added 48h after transfection. Cells were fixed and immunostained for GM130.

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Pincher-generated Nogo-A endosomes mediate growth cone collapse and retrograde signaling

Armela Joset, Dana A. Dodd, Simon Halegoua, Martin E. Schwab

jcb. 2010. 188:271-285 DOI: 10.1083/jcb.200906089.

(in Figure 2: C)

Internalization of Nogo∆20 occurs independently of Dynamin II

PC12 cells were transfected with GFP tagged dynaminK44A (green). 24 hours later, cells were incubated with 100 nM Transferrin-Biotin (red) or 300 nM Nogo∆20-T7 (red) for 30 min at 37°C.

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Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis

Franco J. Vizeacoumar, Nydia van Dyk, Frederick S.Vizeacoumar, Vincent Cheung, Jingjing Li, Yaroslav Sydorskyy, Nicolle Case, Zhijian Li, Alessandro Datti, Corey Nislow, Brian Raught, Zhaolei Zhang, Brendan Frey, Kerry Bloom, Charles Boone, Brenda J. Andrews

jcb. 2010. 188:69-81 DOI: 10.1083/jcb.200909013.

(in Figure 6: Figure 5 and Figure 6)

Ipl1 and Sli15 localization in mcm21-32R

GFP- Ipl1 or Sli15 RFP- Tub1

[ Full Viewer | Article | Figure ]

Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope

Gul Civelekoglu-Scholey, Li Tao, Ingrid Brust-Mascher, Roy Wollman, Jonathan M. Scholey

jcb. 2010. 188:49-68 DOI: 10.1083/jcb.200908150.

(in Figure 6: A)

Perturbation of lamin-B envelope by tail subfragment microinjection

Microinjection of the lamin-B tail dominant negative subfragment disrupts the nuclear lamina and causes defects in mitosis

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Recreation of the terminal events in physiological integrin activation

Feng Ye, Guiqing Hu, Dianne Taylor, Boris Ratnikov, Andrey A. Bobkov, Mark A. McLean, Stephen G. Sligar, Kenneth A. Taylor, Mark H. Ginsberg

jcb. 2010. 188:157-173 DOI: 10.1083/jcb.200908045.

(in Figure 6: C)

Binding of integrin nanodiscs to fibrin

EM images of integrin nanodiscs binding to fibrin. Images taken at a magnification of 65000 with JEOL 1200EX TEM.

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