The JCB DataViewer is a browser-based application for viewing original image files - from various types of microscopes and gel-documentation systems - associated with JCB articles. It is the first browser-based system for viewing multi-dimensional light microscope image data.

Please note: Data for all articles submitted to the JCB after December 1, 2008 will be peer reviewed. All data for articles submitted before this date were uploaded retrospectively and were not peer reviewed.

Featured Images

Quantitative analysis of chromatin compaction in living cells using FLIM–FRET

David Llères, John James, Sam Swift, David G. Norman, Angus I. Lamond

jcb. 2009. 187:481-496 DOI: 10.1083/jcb.200907029.

(in Figure 2: A)

HeLa H2B-2FP mitotic spread

HeLa H2B-2FP mitotic spreads were prepared as described in Material and methods and stained with DAPI.

[ Full Viewer Article Figure ]

Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast

Thibault Courtheoux, Guillaume Gay, Yannick Gachet, Sylvie Tournier

jcb. 2009. 187:399-412 DOI: 10.1083/jcb.200902093.

(in SupFigure 3: A)

rad21-k1 atb2gfpsv40 ndc80gfp cdc11cfp_GFP and phase

rad21-K1 atb2-gfp(MTs) ndc80-gfp (Kts) cell during mitosis. Example of a cell with a merotelic attachment during mitotic progression up to the completion of cytokinesis and cell abscission (bright field picture). One of the daughter cells (upper panel) undergoes a successful second round of mitosis and cytokinesi while the other (lower panel on the right) although alive is delayed in G2.

[ Full Viewer Article Figure ]

From autoinhibition to inhibition in trans: the Raf-1 regulatory domain inhibits Rok-α kinase activity

Théodora Niault, Izabela Sobczak, Katrin Meissl, Gregory Weitsman, Daniela Piazzolla, Gabriele Maurer, Florian Kern, Karin Ehrenreiter, Matthias Hamerl, Ismail Moarefi, Thomas Leung, Oliviero Carugo, Tony Ng, Manuela Baccarini

jcb. 2009. 187:335-342 DOI: 10.1083/jcb.200906178.

(in Figure 4: A)

Rok-alpha (green)and Raf1-reg (red)

Figure 4 - Raf-1reg colocalizes with Rok-alpha and rescues all phenotypes of Raf-1-deficient MEFs. (A) Raf-1 KO MEFs expressing Raf-1reg were identified by staining with antibodies against Raf-1. The localization of Raf-1 and Rok-alpha in migrating MEFs was determined by immunofluorescence.

[ Full Viewer Article Figure ]

Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes

Lit-Hsin Loo, Hai-Jui Lin, Dinesh K. Singh, Kathleen M. Lyons, Steven J. Altschuler, Lani F. Wu

jcb. 2009. 187:375-384 DOI: 10.1083/jcb.200904140.

(in Fig 1: B)

Blue (DNA), Green (lipid droplets), Yellow (adiponectin), Red (PPARgamma), Grayscale (DIC)

Immunofluorescence image showing 3T3-L1 preadipocytes fixed after 9 days of differentiation. Most cells had started to accumulate adiponectin and lipid droplets. However, adiponectin and lipid-droplet levels were negatively correlated on these cells.

[ Full Viewer Article Figure ]

Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor

Preethi Vijayaraj, Cornelia Kröger, Ursula Reuter, Reinhard Windoffer, Rudolf E. Leube, Thomas M. Magin

jcb. 2009. 187:175-184 DOI: 10.1083/jcb.200906094.

(in Figure 3: C)

Intestine of a E9.5 embryo

H&E staining of a E9.5 embryo at high magnification

[ Full Viewer Article Figure ]

Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling

Faraz K. Mardakheh, Mona Yekezare, Laura M. Machesky, John K. Heath

jcb. 2009. 187:265-277 DOI: 10.1083/jcb.200905118.

(in Figure 6: E)

FGFR2 colocalisation with LAMP2 (+EVH1 deleted Spred2)

FGFR2 colocalisation with LAMP2 with or without full-length or EVH1 deleted myc-Spred2.

[ Full Viewer Article Figure ]